Coupling GCxGC with QTOF detection in MS/MS mode

Our application specialist Daniela Peroni has published a new application note

The Agilent 7250 QTOF detector delivers high-resolution MS data, tunable ionization energy and MS/MS capability. Accurate-mass spectra with classic ionization can therefore be complemented with soft ionization spectra as well as MS/MS data for identity confirmation of targets and structure elucidation of unknowns. This makes GC-QTOF valuable for challenging applications that demand confident identification in highly complex matrices (e.g. metabolomics, pesticides analysis).

Comprehensive two-dimensional gas chromatography (GC×GC) is the chromatographic method of choice to unravel high sample complexity. Compared to classic GC, GC×GC grants superior separation power and unmatched peak capacity by coupling two different separation mechanism in a single analysis. Co-elutions are avoided or minimized, granting easier identification and more reliable quantification.

Several examples are available for GC×GC in combination with the QTOF used in scan mode as a HRMS-TOF detector. The elevated number of resolved peaks and the clean 2D spectra, in combination with formula generation based on accurate-mass data, allow more precise and informative profiling. However, up to now GC×GC is hardly used in combination with the QTOF in MS/MS mode. In the full application note the feasibility of GC×GC-QTOF-MS/MS is investigated.

Bedrijfsinformatie

JSB, Joint Analytical Systems Benelux B.V.

Knowledge partner Since 2002 JSB has been active on the European market and in the past ten years JSB positioned itself as a full knowledge partner in chromatography and mass spectrometry solutions. Analytical instruments can be purchased in different places, but solid advice tailored to the needs of your laboratory or process environment is not readily available. However, you can call on JSB for both GC (MS) and LC (MS) techniques specifically for your field of application. We have a full range of custom-made solutions. We consider it our task to offer added value when it comes to instrumentation. These standard or custom-made solutions are often delivered including application. For sample pre-processing to full automation, JSB ensures a turnkey solution of several systems, together with the relevant application. Inspiration meets innovation Your problem is our inspiration, which often results in innovative solutions. JSB presents itself to its customers as a knowledge partner in chromatography. We think very highly of both knowledge and innovation. JSB produces the solution for you in their workshop if no commercial solution is available. That is how JSB always wants to be one step ahead of knowledge. This is also possible because JSB invests in the knowledge and skill of highly-trained staff. Therefore, you can be absolutely sure that JSB is the knowledge partner you have been looking for.

Laatst bijgewerkt: 15-08-2017

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